We narrowed to 3,920 results for: pCMV mammalian

1. pCMV 3xFLAG-ATG3 C264A

   Plasmid

   #129297

   Purpose

   Expresses 3xFLAG-ATG3 C264A in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Cysteine 264 to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcmv3-STING-C88S-FLAG

   Plasmid

   #198187

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C88S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-myc3-Mdm2 D367A

   Plasmid

   #52058

   Purpose

   expression of non-cleavable Mdm2 in mammalian cells

   Depositor

   Trudy Oliver, Tyler Jacks

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 D367A (MDM2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   D367A

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-myc3-Mdm2 p60

   Plasmid

   #52059

   Purpose

   expression of truncated Mdm2 in mammalian cells

   Depositor

   Trudy Oliver, Tyler Jacks

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 p60 (truncated) (MDM2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   p60 (truncated at D367, DVDP motif)

   Promoter

   CMV

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-hA3A-eBE-Y132D

   Plasmid

   #113429

   Purpose

   Expresses hA3A-eBE-Y132D in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y132D

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc3-Mdm2 D367E

   Plasmid

   #52057

   Purpose

   expression of non-cleavable Mdm2 in mammalian cells

   Depositor

   Trudy Oliver, Tyler Jacks

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 D367E (MDM2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   D367E

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV 3xFLAG-ATG3 K243R

   Plasmid

   #129301

   Purpose

   Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 243 to Arginine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-ABEMAX-NLS-P2A_BLAST

   Plasmid

   #135355

   Purpose

   Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-2XNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5D HA-PPM1H S124A/S211A

   Plasmid

   #208370

   Purpose

   Mammalian expression of HA-tagged human PPM1H S124A/S211A

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   HA-PPM1H S124A/S211A (PPM1H Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S124A/S211A

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid

    #201585

    Purpose

    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-542 of DNA polymerase kap…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV 3xFLAG-ATG3 allKR

    Plasmid

    #129298

    Purpose

    Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed all 22 Lysine residues to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV 3xFLAG-ATG3 K295R

    Plasmid

    #129302

    Purpose

    Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 295 to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV10-mFoxP1

    Plasmid

    #35170

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid

    #223508

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y306A; Y384F

    Promoter

    CVM, SP6 and U6

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV7.1_3xFLAG-WWC1

    Plasmid

    #172994

    Purpose

    constitutive expression of FLAG-tagged WWC1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC1 (WWC1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-3*HA-Palmd

    Plasmid

    #216833

    Purpose

    expressing HA tag and the coding sequence of mouse Palmd

    Depositor

    Yuxuan Guo

    Article

    Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

    Insert

    Palmd (mus musculus) (Palmd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6 and U6

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV10-mFoxP1-ES

    Plasmid

    #35169

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pOEM1_pCMV:bmp4-pPH:vsvged

    Plasmid

    #111157

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

    Insert

    hBmp4 (BMP4 Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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