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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHcRed-miR-125b-1

Plasmid

#58989

Purpose

MSCV based expression vector - co-expresses HcRed and human miR-125b-1

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Inserts

HcRed fluorescent protein
miR-125b-1 (MIR125B1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-Gal4UAS-PGK-BFP

Plasmid

#247505

Purpose

GAL4/UAS expression of BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

GAL4/UAS expression of BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Article

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.HaloTag

Plasmid

#244105

Purpose

Bacterial expression of green glucose sensor with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Gab2-MSCV-IRES-GFP

Plasmid

#240126

Purpose

Expresses Gab2 (mouse) in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-IE1

Plasmid

#235541

Purpose

To express tagBFP-2A-IE1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IE1

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-FLAG-coORF63

Plasmid

#235544

Purpose

To express tagBFP-2A-FLAG-coORF8 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ORF63

Use

Lentiviral

Tags

FLAG

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo-MORC3_gRNA_1

Plasmid

#235529

Purpose

gRNA against human MORC3

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

MORC3 (MORC3 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo-MORC3_gRNA_3

Plasmid

#235530

Purpose

gRNA against human MORC3

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

MORC3 (MORC3 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-coICP0

Plasmid

#235536

Purpose

To express tagBFP-2A-coICP0 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagRFP657-2A-coICP0

Plasmid

#235538

Purpose

To express tagRFP657-2A-coICP0 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PKD C1ab

Plasmid

#221755

Purpose

Stable expression of GFP-PKD C1ab in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Prkd1(138-343) (Prkd1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 138-343

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GFP and GST-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

Plasmid

#212809

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

Plasmid

#212804

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-P2A-3xFLAG-Ubc7

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

Plasmid

#212803

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET23a-H6-TEV-AncSZ

Plasmid

#214235

Purpose

Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

Depositor

Neel Shah

Article

Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

Insert

Syk kinase (SYK Human)

Tags

6xHis

Expression

Bacterial

Mutation

Sequence gained through ancestral reconstruction …

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

Plasmid

#212800

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Mutation

HOIP(C885A)

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

Plasmid

#212814

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo(K63R)

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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