Skip to main content
Addgene

We narrowed to 8,865 results for: BLI;

Showing: 3501 - 3550 of 8865 results
  1. pAG Lenti CMV EGFP-HA Puro

    Plasmid
    #236082
    Purpose
    Lentiviral backbone expressing C-terminally HA tagged EGFP, control for empty backbone 236080
    Depositor
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. Ec holB-pET(11a)(ACH-)

    Plasmid
    #237234
    Purpose
    over-express E. coli holB (Pol III delta prime subunit)
    Depositor
    Insert
    holB (delta prime) (holB E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pAG426-GAL-3xFLAG-PolyS

    Plasmid
    #181728
    Purpose
    Galactose inducible expression of 3xFLAG-PolyS
    Depositor
    Insert
    3xFLAG-PolyS
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pAG426-GAL-3xFLAG-PolyA

    Plasmid
    #181726
    Purpose
    Galactose inducible expression of 3xFLAG-PolyA
    Depositor
    Insert
    3xFLAG-PolyA
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pAG426-GAL-3xFLAG-PolyL

    Plasmid
    #181727
    Purpose
    Galactose inducible expression of 3xFLAG-PolyL
    Depositor
    Insert
    3xFLAG-PolyL
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pAG426-GAL-3xFLAG-PolyC

    Plasmid
    #181729
    Purpose
    Galactose inducible expression of 3xFLAG-PolyC
    Depositor
    Insert
    3xFLAG-PolyC
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Insert
    Smt3 (SMT3 )
    Use
    Tags
    HisTag
    Expression
    Mutation
    Promoter
    T7
    Available since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pET15bHis6-pG-MNase

    Plasmid
    #124886
    Purpose
    To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.
    Depositor
    Insert
    protein G-MNase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3.1-hSyncytin-1(deltaFP)

    Plasmid
    #182443
    Purpose
    hSyncytin-1, fusion peptide deletion(320-340)
    Depositor
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    no fusion peptide (320-340)
    Promoter
    CMV-F
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1-hSyncytin-1(C186A)

    Plasmid
    #182450
    Purpose
    hSyncytin-1, fusion deficit and dominant negative mutant (C186A)
    Depositor
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C186A
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pAG Retro EGFP Puro

    Plasmid
    #234463
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pAG Retro EGFP Hygro

    Plasmid
    #234462
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Insert
    Pfdx-E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    fdx
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid
    #221449
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1
    Depositor
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid
    #221450
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1
    Depositor
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid
    #221451
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1
    Depositor
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. pGEX-N-TEV-Twin-Strep

    Plasmid
    #222014
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli
    Depositor
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pUSE-URA3-Sec7-mEYFP

    Plasmid
    #218968
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP
    Depositor
    Insert
    SEC7-mEYFP (SEC7 Budding Yeast)
    Use
    Gene replacement
    Tags
    mEYFP
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pUSE-URA-Sec7-mGold

    Plasmid
    #218967
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold
    Depositor
    Insert
    SEC7-mGold (SEC7 Budding Yeast)
    Use
    Gene replacement vector for yeast
    Tags
    mGold
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid
    #218970
    Purpose
    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2
    Depositor
    Insert
    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    Use
    Gene replacement vector for yeast
    Tags
    moxGFP2
    Expression
    Mutation
    Promoter
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Use
    Tags
    msYFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. MSCV-IRES-tagBFP

    Plasmid
    #219965
    Purpose
    express TagBFP in mammalian cells
    Depositor
    Insert
    TagBFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. lentiGuideSLC35C1-neo

    Plasmid
    #220868
    Purpose
    encodes CRISPR sgRNA targeting human SLC3C1
    Depositor
    Insert
    SLC35C1 (SLC35C1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. icLCT

    Plasmid
    #223179
    Purpose
    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)
    Depositor
    Insert
    cytosolic intestinal lactase protein fragment (Lct Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  27. pXR004_puroR

    Plasmid
    #219820
    Purpose
    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. pCC1R

    Plasmid
    #213497
    Purpose
    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit
    Depositor
    Inserts
    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  29. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  30. MUT_S7-H1

    Plasmid
    #218396
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. AAV-SYN-eGFP-oPRE

    Plasmid
    #218681
    Purpose
    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  32. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid
    #218194
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3
    Depositor
    Insert
    EndoF3
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-329
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. pET-29b(+)_His-CBD-EmEndoF1

    Plasmid
    #218195
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1
    Depositor
    Insert
    EndoF1
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 51-339
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. pAG426-GAL-Prepro-3x-Adan

    Plasmid
    #181733
    Purpose
    Galactose inducible expression of Prepro-3x-Adan
    Depositor
    Insert
    Prepro-3x-Adan
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  35. pAG416-GAL-RNQ1

    Plasmid
    #181706
    Purpose
    Galactose inducible expression of RNQ1
    Depositor
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  36. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211337
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Insert
    m.3075-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  37. pT7CFE1_EVA71_VP12A

    Plasmid
    #212977
    Purpose
    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Insert
    Enterovirus A71 4643 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  38. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  39. pT7CFE1_EVD68_3ABC

    Plasmid
    #212979
    Purpose
    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Insert
    EV-D68 MO/14/18947 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  40. pT7CFE1_EVD68_3ABCD

    Plasmid
    #212981
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts
    Depositor
    Insert
    EV-D68 MO/14/18947 3ABCD
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  41. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  42. pENTR3cDual-Wnk

    Plasmid
    #203976
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Insert
    Wnk (Wnk Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  43. DV.7.1

    Plasmid
    #208584
    Purpose
    Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Insert
    SARS-CoV-2 variant DV.7.1 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  44. HA Display H3_A/Hong Kong/1/1968 Part 4-DO

    Plasmid
    #206518
    Purpose
    CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  45. HA Display H3_A/Hong Kong/1/1968 Part 1-DO

    Plasmid
    #206515
    Purpose
    CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  46. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A nickase
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  47. pEXP1-DEST-Noa1-HIS

    Plasmid
    #191972
    Purpose
    Destination vector containing HIS-tagged NOA1 coding sequence.
    Depositor
    Insert
    Noa1-HIS (NOA1 Human)
    Use
    Synthetic Biology
    Tags
    His
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  48. pcDNA3-PARL-D319N-FLAG C-t

    Plasmid
    #191957
    Purpose
    Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant
    Depositor
    Insert
    PARL D319N (PARL Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D319N
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  49. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  50. KBB96

    Plasmid
    #185113
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains
    Depositor
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Last FXFG domain of Nup1
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 3501 - 3550 of 8865 results