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D67_Ld_111030

Plasmid

#135880

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Publication

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJM110

Plasmid

#134161

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Publication

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 290-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLY110

Plasmid

#130940

Purpose

Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11086_GG_Wheat_live_Cas9_rev

Plasmid

#126882

Purpose

Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

Depositor

Sophien Kamoun

Insert

Wheat_live_Cas9

Use

CRISPR

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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gh110

Plasmid

#106769

Purpose

expression of gRNA targeting B3GALTL (B3GLCT)

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALTL (B3GLCT) (B3GLCT Human)

Expression

Mammalian

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0110

Plasmid

#117573

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Protein kinase superfamily protein {AT1G73460} in A. thaliana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sa_gRNA_Protein kinase superfamily protein {AT1G73460} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB4_p.E110K

Plasmid

#81390

Purpose

Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SERPINB4 (SERPINB4 Human)

Use

Gateway Cloning

Mutation

E110K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEQ1100

Plasmid

#83937

Purpose

express eGFP with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Publication

Child et al J Virol. 2006 Oct;80(20):10173-80.

Insert

EGFP

Tags

6xHIS, V5, and biotinylation signal

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00110

Plasmid

#34265

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4360 (CG4360 Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1101

Plasmid

#29032

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple233 (TNNT1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1108

Plasmid

#29037

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple173 (RAMP3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1109

Plasmid

#29038

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple174 (RAMP3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1103

Plasmid

#29034

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple184 (S100B Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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PH1109

Plasmid

#24830

Depositor

David Baker

Publication

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
L3110

Plasmid

#1529

Depositor

Andrew Fire

Type

Empty backbone

Tags

gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, T7p, lacZN…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pRDAX-110 N-Tag GFP[1] L10 FRB mCherry

Plasmid

#129991

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Expression

Mammalian

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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