We narrowed to 11,422 results for: 112

1. FLAG-EglN2 wt-pBabe-Puro

   Plasmid

   #22704

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-G9WGU4

   Plasmid

   #103112

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   G9WGU4(Cas9 coding gene from Oenococcus kitaharae DSM 17330)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. P2061

   Plasmid

   #186299

   Purpose

   Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

   Inserts

   LexA-HBD-B112
   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   2xLexop-minimalCyc1 and PACT1

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-EglN3 H196A-pcDNA3

   Plasmid

   #22717

   Depositor

   William Kaelin

   Article

   Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

   Insert

   Egg laying Nine - 3 (EGLN3 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   mutant cDNA: point mutation at codon 196 was ins…

   Promoter

   Available since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_NANOS3_cterm

   Plasmid

   #222904

   Purpose

   Cas9/sgRNA plasmid for targeting NANOS3

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-ORF66 C399A-2xStrep

   Plasmid

   #131112

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/TO-ORF66 C289A-2xStrep

   Plasmid

   #131120

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 289 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-ORF66 C244A-2xStrep

   Plasmid

   #131121

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 244 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-EglN2 H358A-pBabe-puro

   Plasmid

   #22705

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated cDNA: point mutation in codon 358 changi…

   Promoter

   Available since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT

    Plasmid

    #60419

    Purpose

    Mammalian Expression of ITSN DH/PH-tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    hITSN1-tgRFPt-SSPB (ITSN1 Synthetic, Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    hITSN 1159-1509 // SSPB Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q

    Plasmid

    #60420

    Purpose

    Mammalian Expression of ITSN DH/PH-tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    hITSN1-tgRFPt-SSPB (ITSN1 Synthetic, Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    hITSN 1159-1509 // SSPB R73Q, Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid

    #223112

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W

    Plasmid

    #211978

    Purpose

    Express gRNA against PDLIM1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting PDLIM1 (PDLIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(PDLIM1_Bru)-PGKpuroBFP-W

    Plasmid

    #211979

    Purpose

    Express gRNA against PDLIM1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting PDLIM1 (PDLIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINRSF

    Plasmid

    #41128

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBiT3.1-C_DPH7:1-452

    Plasmid

    #211276

    Purpose

    Mammalian expression of full-length human DPH7. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DPH7:M1-N452 (WDR85 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiT3.1-N_DPH7:1-452

    Plasmid

    #211202

    Purpose

    Mammalian expression of full-length human DPH7. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DPH7:M1-N452 (WDR85 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINGS

    Plasmid

    #41121

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge