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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mEos2-Actin-N-10

Plasmid

#57341

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PA-mCherry1-Actin-C-18

Plasmid

#57166

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
IDG_CLIC5_OE_1

Plasmid

#161641

Purpose

Overexpresses CLIC5

Depositor

Mike McManus

Insert

CLIC5 (CLIC5 Human)

Tags

V5

Expression

Mammalian

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJA327

Plasmid

#74486

Purpose

superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

superfolder GFP

Expression

Worm

Promoter

myo-3

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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mEos4b-Actin-C-18

Plasmid

#57500

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Actin (ACTB Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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PS-CFP2-Actin-7

Plasmid

#57213

Purpose

Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Article

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSpot-Tag-Actin

Plasmid

#117060

Purpose

For mammalian expression of beta-actin with N-terminal Spot-Tag

Depositor

Felix Hartlepp

Insert

actin beta (ACTB Human)

Tags

Spot-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJA317

Plasmid

#74488

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d1ODC

Use

Lentiviral

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-DRv6

Plasmid

#78628

Purpose

GESTALT V6 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V6

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mKikume-Actin-7

Plasmid

#57322

Purpose

Localization: Actin, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mKikume

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEDL3

Plasmid

#25092

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

Edge detector

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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mKeima-Red-Actin-7

Plasmid

#56013

Purpose

Localization: Actin, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only