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Plasmid#161599PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCfB9359(VIII-1_Markerfree_BackBone)
Plasmid#161600PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9361(XIII-1_Markerfree_BackBone)
Plasmid#161602PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9363(XVI-1_Markerfree_BackBone)
Plasmid#161604PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5b_Ptet
Plasmid#179338PurposegRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC0x_gRNA_Pos5a_Ptet
Plasmid#179337PurposegRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
gSCPL35-mRFP
Plasmid#181960PurposeSCPL35 protein tagged with mRFP, under control of native promoterDepositorInsertSCPL35
UseTagsmRFPExpressionPlantMutationPromoternative SCPL35Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-Ck1-hs2-l-chlo
Plasmid#163938PurposeFor integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha rbcL_trnR region homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_Mprnpo_chlo
Plasmid#163951PurposeL0 part - promoterDepositorInsertMarchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_UBE2:eG-Lt-Ck4
Plasmid#136130PurposeL1 in position 4, mark cell outline with eGFPDepositorInsertp5-MpUBE2:eGFP-Lti6b_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PROM_MpNOP1
Plasmid#136117PurposePROM for cloning with 5UTR_MpNOP1DepositorInsertPROM_MpNOP1 Mapoly0008s0036
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
3TERM_MpNOP1
Plasmid#136119Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_MpNOP1 Mapoly0008s0036
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_NOP1:mS-N7-Ck2
Plasmid#136126PurposeL1 in position 2, example of weak promoterDepositorInsertp5-MpNOP1:mScarletI-N7_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_UBE2:mT-N7-Ck2
Plasmid#136127PurposeL1 in position 2, control of expressionDepositorInsertp5-MpUBE2:mTurquoise2-N7_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_UBE2:mT-N7-Ck3
Plasmid#136128PurposeL1 in position 3, control of expressionDepositorInsertp5-MpUBE2:mTurquoise2-N7_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_UBE2:eG-Lt-Ck3
Plasmid#136129PurposeL1 in position 3, mark cell outline with eGFPDepositorInsertp5-MpUBE2:eGFP-Lti6b_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-HDEL
Plasmid#136097PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_eGFP(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-Lti6b
Plasmid#136099PurposePlasma Membrane targeting protein for C-term fusion with CDS12 without linker.DepositorInsertCTAG_AlaSerGly10xAla_Lti6b
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTAG_linker-mVenus
Plasmid#136101PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_mVenus
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTAG_linker-N7
Plasmid#136102PurposeNuclear targeting peptide for C-term fusion with CDS12 without linker.DepositorInsertCTAG_AlaSer4xAla_N7
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits