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pJW4.23

Plasmid

#62876

Purpose

Bacterial expression of MBP-MEG-3

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-3

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.27

Plasmid

#62877

Purpose

Bacterial expression of MBP-MEG-1 S574A

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1 S574A

Use

Tags

MBP

Expression

Bacterial

Mutation

S574A

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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UAS-Myc-dASUN

Plasmid

#47036

Purpose

Drosophila expression of tagged dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

Myc

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
TV3-mCHY-dASUN

Plasmid

#47034

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

mCHY

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
TV3-eGFP-dASUN

Plasmid

#47033

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pMAH-POLY-eRF1(E55D)

Plasmid

#157925

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEFBOS_CreIRESpuro

Plasmid

#183812

Purpose

Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

Depositor

Ed Stanley

Article

Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

Insert

Cre recombinase

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_G-CEPIA1er

Plasmid

#164590

Purpose

Stable integration and expression of G-CEPIA1er in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

G-CEPIA1er

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_RGECO1.2

Plasmid

#164592

Purpose

Stable integration and expression of RGECO1.2 in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

R-GECO1.2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Use

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDN77

Plasmid

#61347

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS2 and NES IkBa

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Lapronic/pcDNA3.1(-)

Plasmid

#140756

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGH42

Plasmid

#107249

Purpose

Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp

Use

Suicide vector for destined for double homologous…

Tags

mGFPmut3

Expression

Bacterial

Mutation

Promoter

native promoter of sll0617

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19/NLS-mChe

Plasmid

#183162

Purpose

Construct for transient expression of cell nucleus-targeted mCherry in plants.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

NLS-mCherry

Use

Tags

16 aa nuclear localization signal and 16 aa nucl…

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-SpiCee-NLS

Plasmid

#140900

Purpose

mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NLS

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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mNeongreen-aGBD(anillin)

Plasmid

#129633

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding domain of anillin (712-786)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge