We narrowed to 7,348 results for: ALP

1. 3XFLAG-NSarQ27G-VMAn-FKBP

   Plasmid

   #70229

   Purpose

   Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Use

   Tags

   3XFLAG, FK506- and rapamycin-binding protein (FKB…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p3XFlag-CAPN1

   Plasmid

   #60941

   Purpose

   Expresses calpain1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain1 (CAPN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ga13_2-mTq2

   Plasmid

   #112933

   Purpose

   Human Galpha13 tagged with mTurquoise, inserted at residue 128

   Depositor

   Dorus Gadella

   Article

   Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

   Insert

   Galpha13
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HsLSm1_J

   Plasmid

   #146676

   Purpose

   Mammalian Expression of HsLSm1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsLSm1 (LSM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLNHA-C1-HsLSm1_J

   Plasmid

   #146658

   Purpose

   Mammalian Expression of HsLSm1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsLSm1 (LSM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mNeonGreen-α-synuclein

   Plasmid

   #205713

   Purpose

   Mammalian expression of mNeonGreen-alpha-synuclein

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-f:PGC1

   Plasmid

   #1026

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-hRARα.hRXRα

    Plasmid

    #135411

    Purpose

    Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-alpha
    Human retinoid X receptor-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KG#203

    Plasmid

    #110880

    Purpose

    Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6P-HMP1_FABD

    Plasmid

    #186436

    Purpose

    Expresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    HMP1 (F-actin Binding Domain) (hmp-1 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-6P--Dr-aCateninFL

    Plasmid

    #191740

    Purpose

    Expresses FL D rerio catenin in bacteria

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (ctnna1 Zebrafish)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28Man

    Plasmid

    #203399

    Purpose

    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

    Depositor

    David Vocadlo

    Article

    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

    Insert

    man2A
    

    Use

    Tags

    H6

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3H-α-synuclein

    Plasmid

    #205714

    Purpose

    Mammalian expression of 3H-alpha-synuclein

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. α-synuclein-3H

    Plasmid

    #205715

    Purpose

    Mammalian expression of alpha-synuclein-3H

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    thermostable three helix-bundle protein 3H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-αS-2

    Plasmid

    #205706

    Purpose

    Mammalian expression of alpha-synuclein tandem dimer

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein tandem dimer (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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