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psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

Plasmid

#233629

Purpose

Expression of bicistronic reporter with mutated TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

AGAAGG in TOP motif

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Use

Tags

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Use

Tags

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1mut

Plasmid

#203604

Purpose

Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR was mu…

Promoter

SV40

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSi-check2-hRac2 - mut 3'UTR

Plasmid

#97161

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

RAC 3'UTR (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

miR-722 Binding Mutant

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Use

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4Luc-RLuc

Plasmid

#64034

Purpose

a bidirectional reporter vector allowing the study of biscistronic promoter

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Mol Cell Endocrinol. 2010 Apr 12;317(1-2

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0197

Plasmid

#154654

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS Nanoluc expression cassette [NOS::TMV::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0109

Plasmid

#154632

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s LucF expression cassette [35s::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0009

Plasmid

#154616

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0010

Plasmid

#154617

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0091

Plasmid

#154618

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0092

Plasmid

#154619

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_065 Nanoluc expression cassette [MinSyn_065::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0093

Plasmid

#154620

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0100

Plasmid

#154621

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_067 Nanoluc expression cassette [MinSyn_067::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0101

Plasmid

#154622

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_068 Nanoluc expression cassette [MinSyn_068::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0102

Plasmid

#154623

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_069 Nanoluc expression cassette [MinSyn_069::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-NLUC

Plasmid

#98141

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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