We narrowed to 14,584 results for: pET

1. pET LIC cloning vector (2A-T)

   Plasmid

   #29665

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-26b-Nb127D01-HA-His

   Plasmid

   #171566

   Purpose

   Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-HA-His (CXCR2 Human)
   

   Use

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Use

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32a-Trx-His6-TEV_SacB

   Plasmid

   #164588

   Purpose

   Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

   Depositor

   Ernst Schonbrunn

   Insert

   TEV-SacB
   

   Use

   Tags

   Trx-His6 followed by TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-(pos16)GFP

   Plasmid

   #89249

   Purpose

   Expresses GFP with +16 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos16)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    6His, TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28a RUVBL2 Delete

    Plasmid

    #159143

    Purpose

    Expression and protien purification of a mutated RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acid 2-456, Thr127-Glu233 mutated to GPPG

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b/Shigella flexneri IpaA

    Plasmid

    #46178

    Depositor

    Susan Craig

    Article

    Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

    Insert

    IpaA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-MCN-10His-StayGold

    Plasmid

    #211360

    Purpose

    Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28sumo-hRig-I-Full-length

    Plasmid

    #210827

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

    Insert

    hRIG-I (DDX58) Full length protein (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-15b-TEV-C9R

    Plasmid

    #118754

    Purpose

    TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

    Depositor

    Yutaka Kuroda

    Article

    Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

    Insert

    Tobacco Etch Virus protease (NEWENTRY Synthetic)
    

    Use

    Tags

    C9R and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-15b-UbcH5b/2419

    Plasmid

    #11475

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5b (UBE2D2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pET-21a-PE2-His

    Plasmid

    #201973

    Purpose

    Express His-tagged PE2 in bacteria

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    PE2-HIS
    

    Use

    Tags

    His and c-myc NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a(+)-Histag-mCherry (527)

    Plasmid

    #70719

    Purpose

    Expression vector for mCherry purification

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Use

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETM6-At4CL-CmCHS-MsCHI

    Plasmid

    #73394

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-TS2126 RNA Ligase

    Plasmid

    #119941

    Purpose

    Express TS2126 RNA ligase in E. coli

    Depositor

    Takashi Ito

    Article

    Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

    Insert

    TS2126 RNA ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-24d-Flag-SKP1

    Plasmid

    #213690

    Purpose

    Expresses human SKP1 in bacteria cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1 (SKP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET41-MBP-t-A3A-t-His

    Plasmid

    #109231

    Purpose

    Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

    Depositor

    Rahul Kohli

    Article

    Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

    Insert

    human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-28a (+)-Gal4-vp16

    Plasmid

    #171075

    Purpose

    Expresses Gal4-vp16 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and VP16 AD (413-490)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-51b(+) ChemoG 3.1

    Plasmid

    #193802

    Purpose

    Protein production of ChemoG3.1 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG 3.1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP: A206K, T225R

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b+His SUMO PPM1H

    Plasmid

    #207333

    Purpose

    Bacterial expression of His SUMO PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28sumo-MDA5-Full-length

    Plasmid

    #210826

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

    Insert

    MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge