We narrowed to 2,550 results for: CCS

1. tetO-Bcl2-IRES-tdtomato(TET005)

   Plasmid

   #117857

   Purpose

   This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

   Depositor

   C. Ron Yu

   Article

   Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

   Insert

   Bcl2 (Bcl2 Mouse)
   

   Use

   Tta/teto

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-miRFP-6xFLAG

   Plasmid

   #161614

   Purpose

   Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   miRFP-6xFLAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-mRuby3-6xFLAG

   Plasmid

   #161613

   Purpose

   Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   mRuby3-6xFLAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

   Plasmid

   #200977

   Purpose

   Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   D62Q-Q130L-V167L (increase specificity towards he…

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

   Plasmid

   #200980

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTBL210

   Plasmid

   #126451

   Purpose

   Expresses Cas9-XTEN-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV_Actb HMEJ donor

   Plasmid

   #97318

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV_Actb NHEJ donor

   Plasmid

   #97316

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV_Actb MMEJ donor

    Plasmid

    #97315

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBABE-puro-Halo-hMRE11(wt)

    Plasmid

    #208393

    Purpose

    To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-cT3G-cERP2-MAFF

    Plasmid

    #192917

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MAFF (MAFF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-cT3G-cERP2-ELK1

    Plasmid

    #192918

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

    Plasmid

    #117856

    Purpose

    This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-TRE-DIO-tdTomato-f

    Plasmid

    #127092

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tdTomato-f
    

    Use

    AAV

    Tags

    farnesylation signal from c-Ha-Ras

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-PLXNB2-mGAP

    Plasmid

    #86241

    Purpose

    Lentivirus for expression of human Plexin-B2 with mutant GAP domain

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PLXNB2 with point mutations of GAP domain

    Promoter

    hPGK

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMB41

    Plasmid

    #73087

    Purpose

    N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    Avi-GFP tag
    

    Use

    Tags

    Avi and GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge