We narrowed to 5,610 results for: KRAS

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pHBT-NRAS174

Plasmid

#248051

Purpose

To express His6 tag-Avi tag-TEV cleavage site-NRAS (residues 1-174) in E. coli, under the T7 promoter.

Depositor

Shohei Koide

Article

Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

NRAS (NRAS Human)

Tags

His6 tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

includes aa 1-174 only

Promoter

T7

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pT/UBC-mVenus-P2A-NRASG12V

Plasmid

#236074

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V

Promoter

Ubc

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pT/PGK-mVenus-P2A-NRASG12V

Plasmid

#236073

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V

Promoter

PGK

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

Plasmid

#217306

Purpose

sgRNA mcherry + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry

Use

CRISPR

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

Plasmid

#217305

Purpose

sgRNA BFP + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-BFP

Use

CRISPR and Synthetic Biology

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean-pBAD

Plasmid

#54666

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

6xHis and mCerulean

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
TRE-PFE3-HA

Plasmid

#236114

Purpose

Tet inducible PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-HA

Expression

Mammalian

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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U6>XX

Plasmid

#87542

Purpose

Component 1 of 2 for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pYD1

Plasmid

#73447

Purpose

expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

Depositor

Dane Wittrup

Article

Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

Type

Empty backbone

Tags

6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-N1

Plasmid

#54730

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Promoter

none

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pICH41308::hCas9

Plasmid

#49770

Purpose

Level 0 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

hCas9

Use

CRISPR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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