We narrowed to 2,128 results for: Pam

1. phU6-CDX4-gRNA

   Plasmid

   #192511

   Purpose

   Contains gRNA for CDX4

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   CDX4 (CDX4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmU6-CDX1-gRNA

   Plasmid

   #192512

   Purpose

   Contains gRNA for CDX1

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   CDX1 (CDX1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ph7SK-CDX2-gRNA

   Plasmid

   #192513

   Purpose

   Contains gRNA for CDX2

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   CDX2 (CDX2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

   Plasmid

   #160141

   Purpose

   Cre- dependent expression of Sun1GFP for nuclei isolation

   Depositor

   Andreas Pfenning

   Article

   Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

   Insert

   Sun1-GFP (Sun1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N-truncated: removed amino acids 1-207, retained …

   Promoter

   Ef1a

   Available Since

   March 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac-TetO-En1-t2a-Foxa2-hygro

   Plasmid

   #176483

   Purpose

   TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
   

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

   Plasmid

   #139996

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pFOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    FOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164777

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    pGK

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES2-CMV-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164779

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    CMV

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMV2 AAV-RapaInducible-NFZ-HGF

    Plasmid

    #188749

    Purpose

    Rapamycin inducible AAV vector expressing HGF

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Rapamycin inducible

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-4gRNA-GBX2-RFP

    Plasmid

    #192288

    Purpose

    Expresses RFP and four sgRNAs against GBX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    RFP and four sgRNA GBX2 (GBX2 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-ASCL1-LMX1b-NURR1-PuroR

    Plasmid

    #182298

    Purpose

    Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

    Depositor

    Kristen Brennand

    Article

    Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

    Insert

    ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACEMam2-TRPML1

    Plasmid

    #238303

    Purpose

    Expression of human TRPML1 in mammalian cells

    Depositor

    Pravin Mahajan

    Article

    Judith et al Structure, 2025

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    8xHis-3xFLAG-TEV

    Expression

    Mammalian

    Promoter

    hybrid CAG promoter

    Available Since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-ALN

    Plasmid

    #43918

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Insert

    ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Gylcine-serine-glycine (GSG) linker followed by v…

    Promoter

    TRE promoter, Tet-ON

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

    Plasmid

    #192287

    Purpose

    Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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