We narrowed to 146,070 results for: ins;

1. pCMV6-MPP8-W80A-10xHis

   Plasmid

   #194179

   Purpose

   Expresses MPP8-W80A_10xHis

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-W80A inside chromo domain

   Promoter

   CMV

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV GFP-LC3B Q116P G120

   Plasmid

   #129290

   Purpose

   Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBOH-LIC_DDB1:1-1140_delta:396-689

   Plasmid

   #210897

   Purpose

   Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   loop deletion amino acids I396 to D689

   Promoter

   Polyhedrin

   Available since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFBOH-avi-TEV-LIC_DCAF12:1-453

   Plasmid

   #210940

   Purpose

   Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DCAF12:M1-S453 (DCAF12 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R131Q natural variant (VAR_035322)

   Promoter

   Polyhedrin

   Available since

   March 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFBOH-MHL_EML4:250-877

   Plasmid

   #210914

   Purpose

   Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   EML4:I250-A877 (EML4 Human)
   

   Use

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Insect

   Mutation

   K283E natural variant (VAR_031726), I382V Natural…

   Promoter

   Polyhedrin

   Available since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFBD-BirA_ATG16L1:303-607

   Plasmid

   #210942

   Purpose

   Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   ATG16L1:G303-Y607 (ATG16L1 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available since

   March 4, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pFBD-BirA_LRRK2:2141-2527

   Plasmid

   #210901

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBacMam2-DiEx-LIC_LRRK2:2141-2527

   Plasmid

   #210900

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Use

   Tags

   AENLYFQSHHHHHHHHHHDYKDDDDK and M

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   P10 and CMV

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pFBD-BirA_CDC20:161-P477

   Plasmid

   #210888

   Purpose

   Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CDC20:S161-P477 (CDC20 Human)
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFBD-BirA_CDC40:236-578

    Plasmid

    #210885

    Purpose

    Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC40:E236-W578 (CDC40 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAIP4_4REH

    Plasmid

    #234195

    Purpose

    Heterologous protein expression of major latex-like protein in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4REH
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. EYFP Fibronectin

    Plasmid

    #56600

    Purpose

    Localization: Extracellular Matrix, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    Fibronectin
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fibronectin begins amino acids at amino acid 900 …

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pCCG_nit9fl

    Plasmid

    #228409

    Purpose

    Expression of NIT-9 in N. crassa at the his-3 locus

    Depositor

    Robert Haensch

    Insert

    NIT-9
    

    Use

    Protein expression in n. crassa

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid

    #214282

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214278

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid

    #185491

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214279

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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