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AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)

Plasmid

#224189

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13276: pAAV-AiE0779m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3276)

Plasmid

#214842

Purpose

Expression of ChR2(H134R)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787)

Plasmid

#191726

Purpose

AI plasmid ALIAS: AiP12787. Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV-RabiesG

Plasmid

#15785

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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AiP12421 - pAAV-AiE0444h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2421)

Plasmid

#191713

Purpose

AI plasmid ALIAS: AiP12421. Expression of SYFP2 (yellow fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGJJ001

Plasmid

#183619

Purpose

bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-PAmCherry

Plasmid

#133412

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-PAmCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905)

Plasmid

#199774

Purpose

enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

wtGFP plus mutations F46L F64L S65G S72A M153T V1…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hDlx_DIO_ChR2_mCherry

Plasmid

#224459

Purpose

An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

Depositor

Gordon Fishell

Article

Wu et al bioRxiv 2024.07.20.604399

Insert

ChR2-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-GCaMP5g-P2A-PAmCherry

Plasmid

#133411

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-GFP-WPRE

Plasmid

#211490

Purpose

expresses GFP under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMIAII-Cd4

Plasmid

#194401

Purpose

Retroviral expression of mouse CD4

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

Cd4 (Cd4 Mouse)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTK296

Plasmid

#59565

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)

Plasmid

#191729

Purpose

AI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

mTFP1

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GFP-WPRE

Plasmid

#211489

Purpose

expresses GFP under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-hspDRv7_scGstlt

Plasmid

#108870

Purpose

scGESTALT V7 lineage barcode for zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Insert

scGESTALT lineage tracing target V7

Use

Tol2 integration into zebrafish genome

Tags

Expression

Mutation

Promoter

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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