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Showing: 1441 - 1460 of 13372 results

  2. Pb mD3S4-dN

    Plasmid
    #180252
    Purpose
    P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.
    Depositor
    Timothy Springer
    Article
    Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:
    Insert
    D3 of P. berghei HAP2 with N-glycosylation sites mutated
    Use
    Tags
    6His
    Expression
    Mammalian
    Mutation
    N516T, S533N and N539Q
    Promoter
    Available since
    March 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    180252-plasmid-map-sequence-id-357204

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  3. pTJV1ScGm-rpoB

    Plasmid
    #166979
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    gentamycin-3-acetyltransferase (aac(3)-Ia )
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166979-plasmid-map-sequence-id-331330

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  5. pKM54

    Plasmid
    #154157
    Purpose
    Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    8 copies of the Aedes aegypti Rel2 NF-κB site
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154157-plasmid-map-sequence-id-309314

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  6. pKM44

    Plasmid
    #154153
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154153-plasmid-map-sequence-id-309250

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  10. pCRISPR-Cas3

    Plasmid
    #209427
    Purpose
    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Insert
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; crRNA under control of PermE*
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209427-plasmid-map-sequence-id-416703

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  15. pDIRECT_10E

    Plasmid
    #91209
    Purpose
    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91209-plasmid-map-sequence-id-182248

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  18. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Amara, Susan Ingram
    Article
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Insert
    YFPQS
    Use
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pKM7

    Plasmid
    #154164
    Purpose
    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    mCherry
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154164-plasmid-map-sequence-id-309401

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Showing: 1441 - 1460 of 13372 results