Skip to main content
Addgene
Showing: 1441 - 1460 of 10770 results

  3. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    49080-plasmid-map-sequence-id-72210

    Enlarge

  4. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49070-plasmid-map-sequence-id-72197

    Enlarge

  5. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid
    #49079
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49079-plasmid-map-sequence-id-72208

    Enlarge

  6. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid
    #49078
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49078-plasmid-map-sequence-id-72207

    Enlarge

  12. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

    Plasmid
    #128387
    Purpose
    Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
    CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    CLuc394 and NLuc398
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128387-plasmid-map-sequence-id-261539

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

    Enlarge

  15. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

    Enlarge

  16. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Use
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

    Enlarge

  18. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid
    #129421
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-3Flag
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    3X Flag epitope tag
    Expression
    Mutation
    Promoter
    none (3X polyA to mitigate episomal expression)
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129421-plasmid-map-sequence-id-257066

    Enlarge

Showing: 1441 - 1460 of 10770 results