Addgene

We narrowed to 8,793 results for: CAG

Showing: 2161 - 2190 of 8793 results

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pPB-CAG-Tfe3::ERT2-pgk-hph

Plasmid

#48756

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3::ERT2 (Tcfe3 Mouse)

Use

Piggybac

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Expression

Mutation

Promoter

CBA

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

Plasmid

#181742

Purpose

AAV vector for expressing CFL-SN and GCaMP6f

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN-P2A-GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

Plasmid

#133789

Purpose

CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized CjeCas9

Use

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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LZF43: Cre-on CAG-CheRiff-CFP (DIO)

Plasmid

#104114

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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BPK1169: CAG-DmrC-NLS-3xFLAG-P65

Plasmid

#104564

Purpose

Mammalian expression vector for DmrC fused to p65 activation domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-p65

Use

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

Plasmid

#108798

Purpose

Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa234 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV CAG ChR2 E123T T159C 2A tDimer

Plasmid

#85399

Purpose

High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E123T , T159C

Promoter

CAG and ribosomal skip sequence 2A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

Plasmid

#72888

Purpose

fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Insert

Gaussia luciferase, Channelrhodopsin-2, EYFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

Plasmid

#69049

Purpose

lentiviral expression of IKZF2 variant 2 with FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 2

Promoter

CAG

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG APX W41F dimeric (mito matrix)

Plasmid

#40307

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

pea APX W41F (dimeric)

Use

Tags

Flag

Expression

Mutation

W41F mutation.

Promoter

CAG

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

Plasmid

#186044

Purpose

AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

NES-jGCaMP8f

Use

AAV

Tags

NES-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Use

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG::Fnip1-pgk-hph

Plasmid

#48758

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Fnip1 (Fnip1 Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Use

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

Plasmid

#32483

Purpose

Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

ChR2HA-2a-PSAML141F,Y115F:GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

ChR2 2A

Expression

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

Plasmid

#178585

Purpose

Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

DLX2 (DLX2 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(Luc)-GFP

Plasmid

#197887

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-Archon2-KGC-EGFP-ER2

Plasmid

#108421

Purpose

AAV production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAV Cag Flex H2B Gcamp6f reverse

Plasmid

#74155

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6f

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

CAG

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-lck-smMyc-4x6T

Plasmid

#196421

Purpose

Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smMyc

Use

AAV; Dre/rox; astrocyte-selective

Tags

Lck

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

Plasmid

#115142

Purpose

CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Mb3Cas12a

Use

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(1363-1370Mut)-MBP

Plasmid

#190038

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and MBP

Expression

Mammalian

Mutation

1363 RDKDLIES 1370 to GSSGGSSG

Promoter

CMV

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40

Plasmid

#186969

Purpose

PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

Dnmt3a2 (Dnmt3a Mouse, Synthetic)

Use

Piggybac

Tags

Flag

Expression

Mammalian

Mutation

Isoform 2 (residues 220–908)

Promoter

CAG

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

Plasmid

#208361

Purpose

Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

Depositor

David Bennett

Article

Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

Insert

mCherry-TANDEM-PSAM4-GlyR

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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