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Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat-/EE (E318Q; S431E; T432E)
Plasmid#42498DepositorInsertKaiC CII cat-/EE (E318Q; S431E; T432E)
UseTagsExpressionBacterialMutationE318Q; S431E; T432EPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat- (E77Q;E78Q)
Plasmid#41886DepositorInsertKaiC CI cat -(E77Q;E78Q)
UseTagsExpressionBacterialMutationchanged glutamic acid 77 and 78 to glutaminePromotert7 promoterAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21TagsExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F48
Plasmid#23072DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Glutamine, H3 K14…PromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F50
Plasmid#23074DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Arginine, H3 K14R…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F54
Plasmid#23078DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Glutamine, H3 K9Q …PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T22I
Plasmid#1110DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with threonine 22 changed to isoleucine; re…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G81S
Plasmid#1111DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 81 changed to serine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G313S
Plasmid#1114DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 313 changed to serine; regulat…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/E381K
Plasmid#1115DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glutamic acid 381 changed to lysine; r…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A587T
Plasmid#1116DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 587 changed to threonine; regu…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A41V
Plasmid#1117DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 41 changed to valine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSL3 - Brr2 del113 N1104L
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL7 - Brr2 del247 N1104L
Plasmid#90257Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 247 amino acids and N1104LPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pV1338
Plasmid#111443PurposeSolo vector pV1326 + sgScADE2DepositorInsertCaCas9/sgScADE2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsTagsExpressionYeastMutationPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS160 CRISPEY HDV-HDV NotI
Plasmid#114453PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsTagsExpressionYeastMutationPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL MND GFP
Plasmid#36247DepositorInsertGFP (eGFP )
UseLentiviralTagsExpressionMutationPromoterMNDAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2
Plasmid#14126DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
SigmaR1-mNeonGreen
Plasmid#226566PurposeEncodes SigmaR1 protein labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
UseTags6xHis tagExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
UseTagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pNCS mTangerine
Plasmid#91763PurposeBacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)DepositorInsertmonomeric Tangerine
UseTagsno tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
ss-SigmaR1DTM-mNeonGreen
Plasmid#226575PurposeEncodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
UseTagsno tagsExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorInsertPRKN (PRKN Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only