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C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6MYC

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFP1DFB

Plasmid

#185871

Purpose

A parental plasmid for in vivo gene amplification at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90285

Purpose

BB2 with split pyrG marker for homologous integration with linker AB (negative control)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBW100ParsR

Plasmid

#78635

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBW205J105-gfp

Plasmid

#78644

Purpose

pSB3K3 carrying J105-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J105-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW203J116-gfp

Plasmid

#78642

Purpose

pSB3K3 carrying J116-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW202J114-gfp

Plasmid

#78641

Purpose

pSB3K3 carrying J114-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J114-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Pbx2

Plasmid

#74351

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 2 (Pbx2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zhx3

Plasmid

#74366

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc fingers and homeoboxes 3 (Zhx3 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zhx2

Plasmid

#74365

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc fingers and homeoboxes 2 (Zhx2 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zfhx3

Plasmid

#74363

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc finger homeobox 3 (Zfhx3 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tgif1

Plasmid

#74359

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tgif1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

TGFB-induced factor homeobox 1 (Tgif1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Six1

Plasmid

#74358

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

sine oculis-related homeobox 1 (Six1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx3

Plasmid

#74352

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 3 (Pbx3 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBALU3

Plasmid

#69318

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

CFPint-FgF

Expression

Bacterial

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pBALU2

Plasmid

#69317

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

YFPint-FgF

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only