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Plasmid#170624PurposeFor the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539EDepositorInsertLyn11-myc-LOV2GIVe (L)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO chicken-cPLA2-C2-domain
Plasmid#170753PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertPLA2G4A-C2 domain (PLA2G4A Chicken)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Nectin-2
Plasmid#170295PurposeA knock-out vector for the mouse Nectin-2DepositorInsertA gRNA targeting the mouse Nectin-2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (D)
Plasmid#170625PurposeFor the mammalian expression of mLOV2GIVe dark-mimicking mutation C450SDepositorInsertLyn11-myc-LOV2GIVe (D)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjCD69
Plasmid#122279PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjCD69 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjMGA
Plasmid#122280PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjMGA 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjPACS1
Plasmid#122281PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjPACS1 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjSTK4
Plasmid#122282PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjSTK4 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjTP53RK
Plasmid#122283PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjTP53RK 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjYTHDC1
Plasmid#122284PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjYTHDC1 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 AAAA
Plasmid#163579Purposeexpress Dcp2 300 AAAA at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); mutati…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 ΔH1
Plasmid#163578Purposeexpress Dcp2 300 ΔH1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); Lysine…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-ORF68
Plasmid#162659PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cellsDepositorInsertORF68
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-UL52
Plasmid#162663PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cellsDepositorInsertUL52
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 C415A
Plasmid#162633PurposeExpresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C415 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only