We narrowed to 2,753 results for: PGK

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pEHC_PGKneoLox2DTA.2

Plasmid

#112623

Purpose

H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Publication

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Human, Aequorea victoria)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Emerald

Promoter

No Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Publication

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Tags

12X MS2 stem loops

Expression

Mammalian

Promoter

EFS

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Publication

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Publication

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PGK1 gRNA (BRDN0001487049)

Plasmid

#77981

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001146969)

Plasmid

#77982

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

Plasmid

#46593

Depositor

Brian Brown

Publication

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16 Decoy (Mir16-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock

Plasmid

#260207

Purpose

Plasmid encoding for GFP and BFP with a non-targeting CRISPRi gRNA to validate CRISPRi activity

Depositor

Leopold Parts

Publication

Usluer et al Stem Cell Reports. 2023 May 9;18(5):1061

Insert

non-targeting gRNA

Use

CRISPR and Synthetic Biology

Available since

Sept. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep

Plasmid

#260206

Purpose

Plasmid encoding for GFP and BFP with a CRISPRi gRNA targeting GFP to validate CRISPRi activity

Depositor

Leopold Parts

Publication

Usluer et al Stem Cell Reports. 2023 May 9;18(5):1061

Insert

GFP targeting gRNA

Use

CRISPR and Synthetic Biology

Available since

Sept. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-GNAQ Q209P L43A/L44A sgRES

Plasmid

#241924

Purpose

Expresses a GNAQ guide RNA resistant GNAQ Q209P L43A/L44A mutant protein

Depositor

Rizwan Haq

Publication

Shettigar et al Proc Natl Acad Sci U S A. 2026 Jul 28;12

Insert

GNAQ Q209P L43A/L44A sgRES (GNAQ Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Q209P, L43A/L44A

Available since

July 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2728 - pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo

Plasmid

#250260

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

D334N

Promoter

EF-1a

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2726 - pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo

Plasmid

#250258

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(I337D)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

I337D

Promoter

EF-1a

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo

Plasmid

#250259

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(T343V)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

T343V

Promoter

EF-1a

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2724 - pLenti-X1-EF1A-HA-FBXO31(∆F-box)-PGK-Neo

Plasmid

#250256

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box

Promoter

EF-1a

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2725 - pLenti-X1-EF1A-HA-FBXO31(Y309A)-PGK-Neo

Plasmid

#250257

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(Y309A)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Y309A

Promoter

EF-1a

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA-FNIII domain 13069 (VH+VL) synNotch

Plasmid

#247524

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Publication

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Inserts

Expresses a synNotch receptor binding to PTPRZ
Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA-FNIII domain 13069 (VL+VH) synNotch

Plasmid

#247523

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Publication

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Synthetic Biology

Expression

Mammalian

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA-FNIII domain 13062 (VH+VL) synNotch

Plasmid

#247522

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Publication

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA-FNIII domain 13062 (VL+VH) synNotch

Plasmid

#247521

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Publication

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205548

Purpose

Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-p6

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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