We narrowed to 9,182 results for: Pol;

1. pJB53

   Plasmid

   #226309

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
   

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PROM5_Mprnpo_chlo

   Plasmid

   #163951

   Purpose

   L0 part - promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. L1_UBE2:eG-Lt-Ck4

   Plasmid

   #136130

   Purpose

   L1 in position 4, mark cell outline with eGFP

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:eGFP-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1_NOP1:mS-N7-Ck2

   Plasmid

   #136126

   Purpose

   L1 in position 2, example of weak promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpNOP1:mScarletI-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. L1_UBE2:mT-N7-Ck2

   Plasmid

   #136127

   Purpose

   L1 in position 2, control of expression

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mTurquoise2-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. L1_UBE2:mT-N7-Ck3

   Plasmid

   #136128

   Purpose

   L1 in position 3, control of expression

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mTurquoise2-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. L1_UBE2:eG-Lt-Ck3

   Plasmid

   #136129

   Purpose

   L1 in position 3, mark cell outline with eGFP

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:eGFP-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2olig-PR

   Plasmid

   #218917

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-PR
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRY2olig-GR

    Plasmid

    #218919

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-GR
    

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRY2olig-GP

    Plasmid

    #218918

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-GP
    

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1_Cas9-Ck4

    Plasmid

    #136135

    Purpose

    L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:Cas9_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L1_lacZgRNA-Ck2

    Plasmid

    #136137

    Purpose

    L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLS-CMV-mP-Rluc

    Plasmid

    #213507

    Purpose

    CMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assay

    Depositor

    Nadav Ahituv

    Article

    Deng et al Science. 2024 May 24;384(6698):eadh0559.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-PB1-B/BRISBANE/60/2008

    Plasmid

    #187919

    Purpose

    Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Tags

    UNTAGGED

    Promoter

    CMV Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-PA-B/BRISBANE/60/2008

    Plasmid

    #187920

    Purpose

    Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Tags

    UNTAGGED

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. I48 ELP

    Plasmid

    #68937

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

    Depositor

    Andrew MacKay

    Article

    Shi et al J Control Release. 2013 Nov 10;171(3):33

    Insert

    I48 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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