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Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 AvrRpm1
Plasmid#209185PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tagsDepositorInsertAvrRpm1
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL1_1-169
Plasmid#209792PurposeBacterial expression of human PRL-1 without farnesylation motifDepositorInsertPRL-1 (PTP4A1 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL1_1-169_C104D
Plasmid#209793PurposeBacterial expression of human PRL-1 C104D mutant without farnesylation motifDepositorInsertPRL-1 (PTP4A1 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 only; C101DPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169_C104D
Plasmid#209797PurposeBacterial expression of human PRL-3 C104D mutant without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 only; C101DPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FnRPR9-10
Plasmid#182568PurposeHuman fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR8-10
Plasmid#182570PurposeHuman fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2116
Plasmid#170393PurposePlasmid for genetic complementation of LetAB with ΔRing 1 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ48-159)PromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2117
Plasmid#170394PurposePlasmid for genetic complementation of LetAB with ΔRing 2 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ160-279)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2118
Plasmid#170395PurposePlasmid for genetic complementation of LetAB with ΔRing 3 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ280-391)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2119
Plasmid#170396PurposePlasmid for genetic complementation of LetAB with ΔRing 4 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ392-515)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2120
Plasmid#170397PurposePlasmid for genetic complementation of LetAB with ΔRing 5 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ516-633)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2121
Plasmid#170398PurposePlasmid for genetic complementation of LetAB with ΔRing 6 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ634-746)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2122
Plasmid#170399PurposePlasmid for genetic complementation of LetAB with ΔRing 7 LetB mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ747-877)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2114
Plasmid#175961PurposeFor expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ634-746PromoteraraBADAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Monosiga brevicollis CBS-pair domain
Plasmid#166435PurposeExpress pGEX-6P-1 Monosiga brevicollis CBS-pair domainDepositorInsertMonosiga brevicollis CBS-pair domain
UseTagsGSTExpressionBacterialMutationPromoterTac, LacI, AmpAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Naegleria fowleri CBS-pair domain
Plasmid#166437PurposeExpress pGEX-6P-1 Naegleria fowleri CBS-pair domainDepositorInsertNaegleria fowleri CBS-pair domain
UseTagsGSTExpressionBacterialMutationPromoterTac, LacI, AmpAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Toxoplasma gondii CBS-pair domain
Plasmid#166438PurposeExpress pGEX-6P-1 Toxoplasma gondii CBS-pair domainDepositorInsertToxoplasma gondii CBS-pair domain
UseTagsGSTExpressionBacterialMutationPromoterTac, LacI, AmpAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pb mD3S4-dN
Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
UseTags6HisExpressionMammalianMutationN516T, S533N and N539QPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScGm-rpoB
Plasmid#166979PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistanceDepositorInsertgentamycin-3-acetyltransferase (aac(3)-Ia )
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only