Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,501 results for: lenti crispr

Showing: 1441 - 1460 of 7501 results

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide2

Plasmid

#125395

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide1

Plasmid

#125394

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1

Plasmid

#125393

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide4

Plasmid

#125427

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide2

Plasmid

#118201

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3

Plasmid

#118200

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

Plasmid

#118203

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

Plasmid

#118202

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#2

Plasmid

#183120

Purpose

pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EPAS1-2

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti sgRNA(MS2) mCherry

Plasmid

#171053

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

sgRNA(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GALT5catdead-blast

Plasmid

#220869

Purpose

lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Insert

beta-1,3-galactosyltransferase 5 (B3GALT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

encodes D156A and D243A mutations; also, nucleoti…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#3

Plasmid

#174325

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiguide-puro_hNKD2sg#2

Plasmid

#174324

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-pLenti-ACTA2-R179H (LLH647)

Plasmid

#242683

Purpose

Plasmid for expression of ACTA2-R179H cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-R179H_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-R179H

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180M (LLH673)

Plasmid

#242686

Purpose

Plasmid for expression of ACTA2-L180M cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180M_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180M

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180V (LLH669)

Plasmid

#242685

Purpose

Plasmid for expression of ACTA2-L180V cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180V_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180V

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-pLenti-ACTA2-D181G (LLH681)

Plasmid

#242687

Purpose

Plasmid for expression of ACTA2-D181G cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-D181G_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-D181G

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1441 - 1460 of 7501 results

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