We narrowed to 6,085 results for: pcDNA 3.1

1. pcDNA3.1-HNF1A-WT-Flag-UTR

   Plasmid

   #183237

   Purpose

   Expression of FLAG tagged HNF1A

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CD81-(TEV)-Cre

   Plasmid

   #167939

   Purpose

   human CD81-TEV cleavage site-Cre

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD81 (CD81 Human)
   

   Tags

   Cre recombinase and TEV protease cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/V5-His-TOPO-mir17-92

   Plasmid

   #21109

   Depositor

   Joshua Mendell

   Article

   O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

   Insert

   miR-17-92 cluster (MIR17 Human)
   

   Expression

   Mammalian

   Available Since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. mScarletI-T2A-mTq2 pcDNA3.1

   Plasmid

   #230048

   Purpose

   Expresses mScarletI-T2A-mTq2 in human cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   mScarletI-T2A-mTq2
   

   Available Since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. LARG mCherry sspB in pcDNA3.1

   Plasmid

   #90459

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mCherry
   sspB
   

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-N-Myc_1-560 USP7

   Plasmid

   #131244

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 1 - 560. This truncati…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mCherry-CyrCR1.mutant

   Plasmid

   #128746

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Mutation

   5 point mutations disrupting the miR-7 site

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/NDUFS2/myc-His

   Plasmid

   #127486

   Purpose

   Expresses myc-tagged NDUFS2 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1his p66shc S36A (corrected)

    Plasmid

    #32575

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    CMV

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MS2-HA-Hs4E-T_AA

    Plasmid

    #148233

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-nAChr-beta3-YFP

    Plasmid

    #50484

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid

    #50480

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    mCherry fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-N-Myc_535-888 USP7

    Plasmid

    #131248

    Purpose

    Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid

    #131249

    Purpose

    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-FLAG-Dicer-delHelicC

    Plasmid

    #51366

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to HelicC domain deletion

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid

    #112060

    Purpose

    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2
    

    Tags

    Nuclear Export Signal (NES) derived from HIV-1

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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