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pT2U2-YFP

Plasmid

#84544

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U3-YFP

Plasmid

#84547

Purpose

Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U3-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU2T4-YFP

Plasmid

#84548

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2U2T2-YFP

Plasmid

#84549

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU2T4U2-YFP

Plasmid

#84551

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4U2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2U4T2-YFP

Plasmid

#84553

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U4T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU2T2-YFP

Plasmid

#84543

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2-EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_cherry donor_Rosa target (pE002)

Plasmid

#149348

Purpose

Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMC_cherry donor_Polb target (pE038)

Plasmid

#149349

Purpose

Replace donor vector for cherry insertion using the human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-ECFP

Plasmid

#118854

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-ECFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkHD_LSSmOrange

Plasmid

#118860

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

LSSmOrange

Use

Tags

His-tag

Expression

Bacterial

Mutation

A44V,F84L, W145M, I165D, M167L, G202D substituti…

Promoter

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-mTFP1

Plasmid

#118855

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-mTFP1

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 K818R

Plasmid

#111956

Purpose

This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818R mutation

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 K818R

Use

Tags

Expression

Bacterial and Yeast

Mutation

K818R

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 V783L

Plasmid

#111955

Purpose

This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783L mutation

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 V783L

Use

Tags

Expression

Bacterial and Yeast

Mutation

V783L

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 N747D

Plasmid

#111954

Purpose

This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747D mutation

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 N747D

Use

Tags

Expression

Bacterial and Yeast

Mutation

N747D

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge