We narrowed to 2,463 results for: T2A

1. pPB1163

   Plasmid

   #210032

   Purpose

   Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas11-P2A-Cas6-T2A-Cas3
   rtta-T2A-blast
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TO-NFIA.1-SOX9

   Plasmid

   #182310

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NFIA-T2A-SOX9 (NFIA, SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1-copGFP

   Plasmid

   #73030

   Purpose

   Express copGFP

   Depositor

   Kazuhiro Oka

   Insert

   copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAK_EF1a_CasRx_Hygro

   Plasmid

   #134840

   Purpose

   Lentiviral expression of CasRx T2A Hygromycin

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Hygromycin

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-CMV-Nluc

   Plasmid

   #73038

   Purpose

   Express Nluc reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1S-Nluc

   Plasmid

   #73033

   Purpose

   Express Nluc

   Depositor

   Kazuhiro Oka

   Insert

   Nluc
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Truncated EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1S-copGFP

   Plasmid

   #73034

   Purpose

   Express copGFP reporter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Truncated EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1-Nluc

   Plasmid

   #73024

   Purpose

   Express Nluc reporter under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-FlpX-mKate2.0-N2cG

    Plasmid

    #234711

    Purpose

    pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

    Depositor

    David Ng

    Article

    Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

    Insert

    mKate2.0 T2A N2cG
    

    Use

    AAV

    Tags

    T2A

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHM2642

    Plasmid

    #229526

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBHM2643

    Plasmid

    #229527

    Purpose

    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Tags

    t2a-mNeonGreen-mAID_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TC374

    Plasmid

    #122614

    Purpose

    Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Expression

    Worm

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TC476

    Plasmid

    #122629

    Purpose

    Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Expression

    Worm

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TC393

    Plasmid

    #122625

    Purpose

    Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Expression

    Worm

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PRE-Hsp70BbCas9_1.0

    Plasmid

    #190795

    Purpose

    PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    PRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBHM2641

    Plasmid

    #229525

    Purpose

    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    CRISPR

    Tags

    t2a-mNeonGreen-AID*

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    TEF1

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBHM2647

    Plasmid

    #229989

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBHM2648

    Plasmid

    #229990

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBHM2649

    Plasmid

    #229991

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge