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Plasmid#229388PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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AAV-U6/TO-shEsr1-CMV-TetR-eGFP
Plasmid#233298PurposeDOX-inducible U6 driven shRNA against mouse Esr1DepositorInsertEsr1 shRNA (Esr1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A
Plasmid#231520PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 61 Q to A of the HMG d…PromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Monbr-Sox-like
Plasmid#231530PurposeExpresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Salhel-I-K57E
Plasmid#231519PurposeExpresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generationDepositorInsertSalhel-I HMG K57E flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 57 K to E of the HMGPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Salro-Sox-like
Plasmid#231531PurposeExpresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-TetOn-mCherry-OAZ1_FS-YFP
Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem
Plasmid#229795PurposeBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2DepositorInsertTRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PTet-MGapt-UTR1-deGFP
Plasmid#227647PurposepTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
UseTagsExpressionYeastMutationDHFR (P66L), Rho1 (C17R)PromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-NLS-VSV
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag43-iCAM-tether
Plasmid#205211PurposeThis vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43DepositorInsertsynCAM iCAM1 tether and Lag43 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-CSRNP2-P2A-mCherry
Plasmid#203867PurposeTet-inducible lentiviral plasmid expressing CSRNP2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertCSRNP2 (CSRNP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-PBX4-P2A-mCherry
Plasmid#203864PurposeTet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertPBX4 (PBX4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTT]-nLuc
Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only