We narrowed to 4,957 results for: mcs

1. pTBL3342 PB-PEmax-puro

   Plasmid

   #226674

   Purpose

   PiggyBac cargo vector encoding PEmax and puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   PEmax
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+ dgat1a-FLAG

   Plasmid

   #232143

   Purpose

   Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1a (dgat1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRII-TOPO-dgat1b in situ probe

   Plasmid

   #232138

   Purpose

   zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1b
   

   Use

   In vitro transcription

   Promoter

   SP6/T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL3328 PB-sfGFP-WPRE-target

   Plasmid

   #226676

   Purpose

   PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   sfGFP-WPRE-site3target
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBL3428 MTK234-LacO-BtoApegRNA

   Plasmid

   #226669

   Purpose

   MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   hU6-LacOS-BtoApegRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-LacOS

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL3331 MTK234-TetO-AtoBpegRNA

   Plasmid

   #226668

   Purpose

   MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   hU6-TetO-AtoBpegRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-TetO

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL3401 BtoApegRNA-sfGFP

   Plasmid

   #226667

   Purpose

   PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   BtoA pegRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSyn-GEM

   Plasmid

   #187648

   Purpose

   Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Insert

   PfV
   

   Use

   AAV

   Tags

   HaloTag and SNAP-tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hSyn-tractin

    Plasmid

    #187649

    Purpose

    Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    F-tractin
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

    Plasmid

    #214614

    Purpose

    adapter for RTK split TEV recruitment assays

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    3xSH2 domains of GRB2-CTEV (GRB2 Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3_ERBB4-NTEV-tcs-GV-2xHA

    Plasmid

    #214613

    Purpose

    ERBB4 receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    ERBB4-NTEV (ERBB4 Human)
    

    Tags

    TEV-Gal4-VP16-2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_EGFR-NTEV-tcs-GV-2xHA

    Plasmid

    #214610

    Purpose

    EGFR receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    EGFR-NTEV (EGFR Human)
    

    Tags

    TEV-Gal4-VP16-2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. NK621

    Plasmid

    #196405

    Purpose

    Strong expression of firefly luciferase codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP24_T24_pRham-ilvA-relE_RBS3

    Plasmid

    #201532

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3
    

    Use

    Synthetic Biology

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT-ChemoG-NAD-NLS3x

    Plasmid

    #193822

    Purpose

    CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-NAD-NLS3x
    

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

    Plasmid

    #193828

    Purpose

    CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-NAD-NLS3x
    

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

    Plasmid

    #193829

    Purpose

    CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    [4xCox8]-ChemoL-NAD
    

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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