161,152 results

1. DRH334: FCK-QuasAr2-mO2

   Plasmid

   #51692

   Purpose

   Fluorescent reporter of membrane voltage with improved sensitivity

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2-mOrange
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rich-pT7

   Plasmid

   #108445

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Q23

   Plasmid

   #40261

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSGEMKv10.1

   Plasmid

   #85704

   Purpose

   Xenopus laevis oocyte expression of Kv10.1

   Depositor

   Luis Pardo

   Article

   Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. sUPRa

   Plasmid

   #223242

   Purpose

   sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

   Depositor

   Colin Akerman

   Article

   Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

   Insert

   sUPRa
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Custom

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

   Plasmid

   #173899

   Purpose

   The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

   Depositor

   Jason W Chin

   Article

   Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

   Inserts

   ThrRS(v1.0)
   tRNA(v2.0)CUA
   pduX
   EF-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCM66T: trimmed pCM66 backbone

   Plasmid

   #74738

   Purpose

   Small IncP-based broad host range replicating vector

   Depositor

   Mary Lidstrom

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAPDH

   Plasmid

   #155622

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-hChR2(H134R)-mCherry

   Plasmid

   #26975

   Purpose

   AAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-2xFLAG-SREBP-1a

    Plasmid

    #26801

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1a (SREBF1 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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