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pDonor_attP-Ef1a-Puro-P2A-mCherry

Plasmid

#247159

Purpose

attP donor plasmid expressing puromycin resistance and mCherry

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHS_Nova

Plasmid

#239831

Purpose

NovaIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 and swap o…

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has Service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Promoter

synapsin

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-AAVS1_B3903c_attP

Plasmid

#182144

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

AAVS1_B3903c_attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-AAVS1_A3786c_attP

Plasmid

#182143

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

A3786c-attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LbNOX-FLAG

Plasmid

#218631

Purpose

This plasmid contains LbNOX under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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