Addgene

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pHR Lck-TagBFP

Plasmid

#171034

Purpose

TagBFP-tagged Lck kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Lck

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31

Plasmid

#165845

Purpose

_C31 integrase CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 integrase

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hHDAC6

Plasmid

#21099

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 6 (HDAC6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pEBFP2

Plasmid

#165831

Purpose

EBFP2blue fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EBFP2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDmAlfaTub84b

Plasmid

#165781

Purpose

Drosophila Alpha Tubulin promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

AlfaTub84b

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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FGD5

Plasmid

#28109

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

FGD5:HPC09X-H08:C212499 (FGD5 Human)

Use

Tags

His-tag with TEV site

Expression

Bacterial

Mutation

contains amino acid residues 889-1304.

Promoter

Available since

March 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pHR Src-TagBFP

Plasmid

#171036

Purpose

TagBFP-tagged Src kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV128-Rluc-RRE_O

Plasmid

#147109

Purpose

Mammalian Expression of Rluc-RRE

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

Rluc-RRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBSR

Plasmid

#165838

Purpose

blasticidin S resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

bsr

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL80

Plasmid

#165825

Purpose

GAL4 inhibitor coding sequence (CDS)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL80

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQS

Plasmid

#165826

Purpose

QF inhibitor coding sequence (CDS)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QS

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmActin5c

Plasmid

#165777

Purpose

Drosophila Actin 5C promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Actin5c

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFCU1

Plasmid

#165842

Purpose

5-fluorocytosine sensitivity marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

FCU1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Tags

Expression

Worm

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJK_proK17_amiE

Plasmid

#59837

Purpose

Constitutively expresses AmiE with proK17 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

proK17

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGAD424-AP-4 σ4

Plasmid

#198328

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b_SNAP-ecDHFRmt

Plasmid

#187109

Purpose

Expression of SNAP-ecDHFRmt fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFRmt

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

ecDHFR-R44L-H45Q

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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prrnBT1_STD

Plasmid

#165803

Purpose

Bacterial rrnB gene T1 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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prrnBT2_STD

Plasmid

#165804

Purpose

Bacterial rrnB gene T2 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHPH

Plasmid

#165839

Purpose

hygromycin B resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

hph

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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