Addgene

We narrowed to 6,446 results for: poly

Showing: 2221 - 2250 of 6446 results

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yFusionRed

Plasmid

#111916

Purpose

yeast optimized FusionRed

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

yFusionRed

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

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pLTE EGFP

Plasmid

#67044

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EGFP

Use

Cell-free expression

Tags

Myc

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
p5mCherry

Plasmid

#92023

Purpose

mCherry reporter plasmid with lac operator in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

mCherry

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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p5sfGFP

Plasmid

#92024

Purpose

sfGFP reporter plasmid with lac operator in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

sfGFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLz48

Plasmid

#226322

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

strep-CsoS1A

Use

Tags

Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBz12

Plasmid

#226321

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-Rubisco

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLz92

Plasmid

#226317

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-CsoS2B

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Phusion

Plasmid

#206210

Purpose

Inducible expression of DNA polymerase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

Phusion DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSC101-pT7-T3RNAP

Plasmid

#140872

Purpose

T7 promoter driven synthesis of T3 RNA polymerase

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

T3 RNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL-GFP kan

Plasmid

#122060

Purpose

galactose inducible expression of GFP in yeast. marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Insert

green fluorescent protein

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p5-79

Plasmid

#118087

Purpose

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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p6-8

Plasmid

#118089

Purpose

rapa-T7- mRNA(GFP)

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKK01

Plasmid

#64744

Purpose

Yeast expression plasmid: phaA, phaB, phaC

Depositor

Jens Nielsen

Article

Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

Insert

phaA, phaB, phaC

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pHR_AXP_hrGFP

Plasmid

#84613

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCJ162

Plasmid

#175055

Purpose

lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

lox66::gmR::lox71

Use

Unspecified

Tags

Expression

Mutation

Promoter

n/a

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_XPR2_hrGFP

Plasmid

#84614

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGWT35S-MpPHOT-Citrine

Plasmid

#220106

Purpose

Transient expression of MpPHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-Citrine

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCNPpo6

Plasmid

#46086

Depositor

Raymond Monnat

Article

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Insert

PpoI

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-H-mScarlet3-3xFLAG-Rab5

Plasmid

#225243

Purpose

Mammalian expression of iPASnFR-H fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-STS

Plasmid

#35949

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

Stilbene synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

LacPMod

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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p11U1ADb

Plasmid

#27384

Depositor

David Bartel

Article

Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

Insert

U1A A1-98 Y31H/Q36R (SNRPA Human)

Use

Tags

Expression

Bacterial

Mutation

A1-98 Y31H/Q36R double mutant

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pR5-28

Plasmid

#22289

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
CUP Ent2 YFP p316

Plasmid

#15593

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

ENT2 (ENT2 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RRVH

Plasmid

#63623

Purpose

T3-R5-RRVH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3 RRVH RNAP

Use

Tags

Expression

Mutation

T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

Promoter

T5/Lac

Available since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGST1-TOM1-VHS

Plasmid

#44423

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

TOM1-VHS (1-152) (TOM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Use

Tags

Expression

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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