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pComb3X-goldengatefriendly

Plasmid

#249256

Purpose

pComb3X plasmid engineered for golden gate insertion of antibodies

Depositor

Sarel Fleishman

Publication

Tennenhouse et al bioRxiv 2025.12.10.693474

Type

Empty backbone

Use

Golden gate vector

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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EKv413(Gold Cas-guide insert4 2 gene version)

Plasmid

#248342

Purpose

gRNA insert vector for Golden Gate Assembly, 2 gene or 4 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv414(Gold Cas-guide insert6 3 gene version)

Plasmid

#248343

Purpose

gRNA insert vector for Golden Gate Assembly, 3 gene or 6 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv376(gRNAscaffold-tRNA for cloning)

Plasmid

#248333

Purpose

Plasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assembly

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Insert

gRNAscaffold-tRNA

Use

CRISPR

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pEVBC1_moxBFP

Plasmid

#248345

Purpose

Blue fluorescent protein control for Fluorescent Protein Library

Depositor

Calin Plesa

Publication

Benabbas et al bioRxiv 2026.03.01.706892

Insert

moxBFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P_LacUV5

Available since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-His-zfAAA-T7RNAP

Plasmid

#247374

Purpose

The plasmid encode N-terminal his tagged zf438-T7 RNAP that pair with pzfAAA-d1T7 promoter

Depositor

Sebastian Maerkl

Publication

Lee et al bioRxiv 2025.06.18.660355

Insert

His-zfAAA-T7RNAP

Tags

His

Expression

Bacterial

Promoter

T5-lacO

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21(+)-His-zf438-NB23

Plasmid

#247372

Purpose

Express zf438-NB23 that bind to SARS-CoV-19 Spike protein cooperatively with NB15-T7 RNAP.

Depositor

Sebastian Maerkl

Publication

Lee et al bioRxiv 2025.06.18.660355

Insert

His-zf438-NB23

Tags

6xHis

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NSK477 SFFV-mCherry-SGc(Smn2ex7.os13-72bp.lowBG.16bp loop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247291

Purpose

Low baseline modulADAR sensor for SMN exon 7

Depositor

Xiaojing Gao

Publication

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-Smn2ex7.os13-72bp.lowBG.16bp loop-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK465 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247290

Purpose

Low baseline modulADAR sensor for NOT SCN1a exon 20N

Depositor

Xiaojing Gao

Publication

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK250 CMV-TO-SCN1A (exons 20, 20N, 21)

Plasmid

#247285

Purpose

Expresses human SCN1a exons 20, 20N, 21

Depositor

Xiaojing Gao

Publication

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SCN1a exons 20, 20N, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK249 CMV-TO-SCN1A (exons 20, 21)

Plasmid

#247284

Purpose

Expresses human SCN1a exons 20, 21

Depositor

Xiaojing Gao

Publication

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SCN1a exons 20, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPB41L4A-AS1

Plasmid

#242748

Purpose

Expresses EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Expression

Mammalian

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-eGFP

Plasmid

#242945

Purpose

Encodes a fragment of eGFP for generating dsRNA for use as a control in RNAi experiments

Depositor

Erin Davies

Publication

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

eGFP

Use

RNAi

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZNpF-∆11cag

Plasmid

#245370

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11cag (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆7cg

Plasmid

#245371

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7cg (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆7

Plasmid

#245367

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆8

Plasmid

#245368

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆8 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

synthetic 8-bp deletion

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆48∆11

Plasmid

#245369

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆48∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus synthetic 48-bp up…

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-wt

Plasmid

#245364

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆59

Plasmid

#245365

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Publication

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆59 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

59-bp deletion, D78Vfs*6

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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