165,528 results

1. pAAV_Ef1a_DIO_hTyrHA_minBack

   Plasmid

   #205286

   Purpose

   Induces NM-like pigment accumulation in catecholaminergic neurons

   Depositor

   Matthias Prigge

   Insert

   hTyrosinase
   

   Use

   AAV

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pODN-mNG-ANLN

   Plasmid

   #183834

   Purpose

   Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN homology arms with mNeonGreen-linker (ANLN Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF3124

   Plasmid

   #144600

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTETRIS-cargo

   Plasmid

   #126033

   Purpose

   Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

   Depositor

   Mauro Calabrese

   Article

   Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

   Type

   Empty backbone

   Use

   Luciferase; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGT-B1

   Plasmid

   #122579

   Purpose

   MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLexM-SLC3A2

   Plasmid

   #197994

   Purpose

   Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Please see depositor comments below

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmScarlet_C1

   Plasmid

   #85042

   Purpose

   Mammalian expression of mScarlet

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZac2.1 gfaABC1D-Glt1-eGFP

    Plasmid

    #176862

    Purpose

    Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Glt1-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge