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pCMV-SlugCBE-NNG-P2A-puro

Plasmid

#214355

Purpose

Expresses SlugCBE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCBE-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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MMLV-RT for Split-PE (pEH45)

Plasmid

#190105

Purpose

Expresses MMLV-RT (full-length, from PE2) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT

Tags

bpNLS

Expression

Mammalian

Mutation

RT pentamutant from PE2 (D200N, T306K, W313F, T33…

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-Y207F+W275L

Plasmid

#196022

Purpose

Expresses CRKL Y207F + W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W275L

Plasmid

#196019

Purpose

Expresses CRKL W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-MCS-P2A-GFP-PuroR

Plasmid

#107186

Purpose

Base plasmid for cloning gene duplication alleles with eGFP reporter.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Type

Empty backbone

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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B2094_CRY2olig-miRFP

Plasmid

#215114

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-miRFP (CRY2 Mustard Weed)

Tags

miRFP

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(1)

Plasmid

#73554

Purpose

vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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