Addgene

We narrowed to 6,032 results for: WUS;

Showing: 2251 - 2280 of 6032 results

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Use

Tags

Fis1

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-cytoB5RR

Plasmid

#182577

Purpose

Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-cytoB5RR

Use

Tags

LifeAct and cytoB5RR

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Use

Tags

EPEA and Flag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV2

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMB-SaCas9

Plasmid

#105998

Purpose

SaCas9 expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Insert

Cas9 nuclease

Use

CRISPR and Lentiviral

Tags

none

Expression

Mammalian

Mutation

none

Promoter

short EF1alpha

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Use

Tags

24×Broccoli

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-IRESWT-Gbetagamma ONE-GO

Plasmid

#204339

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-i1 ONE-GO

Plasmid

#204333

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i1 ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-A

Plasmid

#177786

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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MTK2_007

Plasmid

#123702

Purpose

Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pEF1a

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-oA ONE-GO

Plasmid

#204335

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-oA ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDual-IN-SMN1

Plasmid

#215721

Purpose

dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN1 EXON 7 (SMN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1

Plasmid

#39281

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1

Plasmid

#39282

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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