We narrowed to 9,766 results for: 158

1. pJK560

   Plasmid

   #71585

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed arginine-232 to histidine

   Promoter

   T7 promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK504

   Plasmid

   #71584

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-357 to tyrosine

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVD518

   Plasmid

   #66158

   Purpose

   Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

   Depositor

   James Kaper

   Article

   Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

   Insert

   tdh1
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-myc-Rem2 R236A/L263A

   Plasmid

   #51589

   Purpose

   expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R236A and L263A; silent mutations at shRNA 1 and …

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-myc-Rem2 S69A/S334A

   Plasmid

   #51588

   Purpose

   expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S69A and S334A; silent mutations at shRNA 1 and 2…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRmHa3-dKDM4A-H195A-HA2FL2

   Plasmid

   #20235

   Depositor

   Jerry Workman

   Article

   Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

   Insert

   dKDM4A (Kdm4A Fly)
   

   Use

   Tags

   FLAG2 and HA2

   Expression

   Insect

   Mutation

   changed His 195 to Ala

   Promoter

   Available since

   March 19, 2009

   Availability

   Academic Institutions and Nonprofits only

7. piggyBAC-5xTAPE-1-BlastR

   Plasmid

   #175808

   Purpose

   Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Insert

   5xTAPE-1 construct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pscALPS- SIV-MAC251 vpx

   Plasmid

   #115810

   Purpose

   Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMAC251 Vpx
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-CBE6b

    Plasmid

    #215820

    Purpose

    Express CBE6b (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-CBE6d

    Plasmid

    #215822

    Purpose

    Express CBE6d (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2-cryR;cldn5bP1Egfp

    Plasmid

    #90158

    Purpose

    contains cldn5b specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    cldn5bP1Egfp (cldn5b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPM-D4- miR30-L1221

    Plasmid

    #115846

    Purpose

    Control shRNA KD vector

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    control shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. SpCas9-CBE6c

    Plasmid

    #215821

    Purpose

    Express CBE6c (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge