We narrowed to 2,550 results for: CCS

1. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

   Plasmid

   #224123

   Purpose

   Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-mutant (Q20A_Y23A_Y24F_F35A)
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Q20A_Y23A_Y24F_F35A in La(SSB)1-194

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDR-FOXL2-T2A-tdTomato-PuroTK

   Plasmid

   #192892

   Purpose

   HDR donor for inserting FOXL2-T2A-tdTomato reporter

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2-tdTomato (FOXL2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   T2A-tdTomato fluorescent reporter

   Promoter

   PGK

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

   Plasmid

   #126480

   Purpose

   Expresses Cas9-GSAlinker-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   GSA linker
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-DIO-ChR2(H134R)-eYFP

   Plasmid

   #127090

   Purpose

   Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Has Service

   AAV PHP.eB

   Insert

   Channelrhodopsin-2
   

   Use

   AAV

   Tags

   eYFP (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Humanized ChR2 gene with histidine 134 changed to…

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGIPz-PD-L1 WT

   Plasmid

   #121486

   Purpose

   2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

   Plasmid

   #132667

   Purpose

   Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   4xHRE_YB TATA

   Available since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

   Plasmid

   #85798

   Purpose

   donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   homology arms for knock-in into first intron

   Promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-FOS

   Plasmid

   #192924

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOS

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOS (FOS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

    Plasmid

    #208395

    Purpose

    To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DB1delta/DB2delta

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgRNA-Cre-GpNluc-sgControl

    Plasmid

    #208383

    Purpose

    This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgControl (TP53BP1 intron) (Trp53bp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-UBC-S2-cAMPr

    Plasmid

    #160723

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-cAMPr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

    Plasmid

    #85800

    Purpose

    donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    homology arms for knock-in into first intron

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-cT3G-cERP2-HOPX

    Plasmid

    #192923

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    HOPX (HOPX Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-S3-ExRaiAKAR

    Plasmid

    #160724

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-ExRaiAKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR-53BP1 1-1972 28A

    Plasmid

    #53447

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    I914T mutation; All 28 SQ/TQ motifs in N-term of …

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. AF20-double-tag

    Plasmid

    #108389

    Purpose

    Express human PC1

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

    Plasmid

    #126477

    Purpose

    Expresses Cas9-5xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    5xFlag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed codons to reduce repetitive sequences.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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