Addgene

We narrowed to 2,368 results for: GCG

Showing: 1501 - 1520 of 2368 results

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon5

Plasmid

#217456

Purpose

For TP53 knockout targeting exon 5 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-VIM

Plasmid

#227301

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of VIM (VIM Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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POLR2A-N CRISPR pX330

Plasmid

#124495

Purpose

A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A targeting CRISPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiCh2-2

Plasmid

#202444

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

Chromosome 2 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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gh69

Plasmid

#106740

Purpose

expression of gRNA targeting B3GNT3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT3 (B3GNT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001162598)

Plasmid

#75577

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145598)

Plasmid

#80173

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001146586)

Plasmid

#77947

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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STK17A gRNA (BRDN0001149480)

Plasmid

#77916

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK17A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK17A (STK17A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gDNMT1.Cas9-T2A-GFP

Plasmid

#170362

Purpose

Plasmid used for Crispr/cas9 based disruption of human DNMT1.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKO_LacZ_sgRNA

Plasmid

#74179

Purpose

lentiviral vector expressing sgRNA targeting LacZ

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

LacZ sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA3

Plasmid

#195197

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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