We narrowed to 1,587 results for: Tef

1. pcDNA 3.1 - circMAN2A1

   Plasmid

   #198837

   Purpose

   This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

   Depositor

   Stefan Stamm

   Article

   Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

   Insert

   circular mannosidase alpha class 2A member 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   circular RNA composed by 2 exons

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC28-HiBIT-WDR5

   Plasmid

   #200883

   Purpose

   Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HiBIT-WDR5 (WDR5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTVL2-BIRC2-BIR3

   Plasmid

   #196367

   Purpose

   protein expression of the GST-tagged BIR3 domain

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Front Cell Dev Biol. 2022 May 26;10:8865

   Insert

   BIRC2 BIR3 domain
   

   Use

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   contains only S260-Y352

   Promoter

   T7

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAFMW-Nbr[R272A,K276A, R280A,K284A]

   Plasmid

   #188599

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBY011-AMN1ko

   Plasmid

   #183099

   Purpose

   S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

   Depositor

   Gabor Balazsi

   Article

   Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

   Insert

   AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
   

   Use

   Tags

   KanMX

   Expression

   Bacterial and Yeast

   Mutation

   only includes external sequences (504 bp each) as…

   Promoter

   URA3, TEF1

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human USP7-3X FLAG

   Plasmid

   #225334

   Purpose

   Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human USP7_H456A-3X FLAG

   Plasmid

   #225335

   Purpose

   Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   H456A

   Promoter

   EF1a

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

    Plasmid

    #194171

    Purpose

    Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-Associated Protein Tau 0N4R
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Use

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

12. FRT_htr2c(c)-GFP

    Plasmid

    #79677

    Purpose

    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    serotonin receptor 2C (HTR2C Human)
    

    Use

    Tags

    EGFP in Frame

    Expression

    Mammalian

    Mutation

    splice site of exon Vb in consensus

    Promoter

    CMV

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pDIV313

    Plasmid

    #204956

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus fumigatus U3 promoter, Aspergillus ni…

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-SFB-USP7

    Plasmid

    #99393

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    USP7 (USP7 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOGS539_GLuc-ns

    Plasmid

    #226719

    Purpose

    Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV14_NHP2L1(15.5kD)

    Plasmid

    #73065

    Purpose

    expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NHP2L1 (15.5kD) (SNU13 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTXB1-EXO1b D173A

    Plasmid

    #68269

    Purpose

    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

    Depositor

    Stefano Ferrari

    Article

    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    catalytically-dead D173A. Please see depositor co…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET26b(+)-ATI_QconCAT_1

    Plasmid

    #163957

    Purpose

    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

    Depositor

    Stefan Tenzer

    Article

    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

    Insert

    ATI QconCAT
    

    Use

    Tags

    polyhistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_ZKSCAN1_Tau7-12WT

    Plasmid

    #194166

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 7-12 WT
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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