We narrowed to 1,568 results for: pet-28a

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Kindlin

Plasmid

#186135

Purpose

For expression of recombinant kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1362

Plasmid

#166860

Purpose

Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp7 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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SpyTag003-OspC

Plasmid

#159998

Purpose

Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag003-OspC (20-209) C129S

Tags

His6-tag and Thrombin cleavage site

Expression

Bacterial

Mutation

C129S mutation improves expression level.

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SIMx6

Plasmid

#116938

Purpose

Bacterial expression vector for 6 sim

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

6 sim

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Tags

His tag with TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Expression

Bacterial

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
Human USP30 (64-502, construct 13i, C77A, MG-31-28)

Plasmid

#110747

Purpose

Bacterial expression of human USP30 (construct 13i)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-GST-3C

Expression

Bacterial

Mutation

C77A, F348D, M350S, I353E + insertion deletion: 6…

Promoter

T7

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

Plasmid

#243822

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551 R1598E F1658E

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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E547* FL USP7

Plasmid

#229001

Purpose

E547-mutant of human USP7

Depositor

Irina Bezsonova

Insert

USP7 1-546 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJK287

Plasmid

#71120

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCeSR beta deltaN

Plasmid

#13728

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (R186.3 Nematode)

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 38-240 of C. elegans SRβ with …

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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