165,528 results

1. PPP1CA

   Plasmid

   #155843

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPP1CA (PPP1CA Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT1 KI gRNA1

   Plasmid

   #92312

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c5X-GLP-1

   Plasmid

   #66997

   Purpose

   Expresses malE-GLP-1 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-GLP-1
   

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGG-AH-VRT-A2

   Plasmid

   #163703

   Purpose

   Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)

   Depositor

   Cristobal Uauy

   Article

   Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.

   Inserts

   Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
   Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
   

   Expression

   Plant

   Promoter

   Rice - Os Actin promoter and Triticum polonicum V…

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #219782

   Purpose

   CaST-IRES for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

    Plasmid

    #81253

    Purpose

    Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    TetR and LacI
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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