We narrowed to 7,239 results for: ALP

1. plKO.1-DNAJC5-ShRNA

   Plasmid

   #205729

   Purpose

   Knockdown of DNAJC5

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   shRNA targeting DNAJC5 (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZIP-hCMV-ZsGreen-P2A-Puro-NT4

   Plasmid

   #123328

   Purpose

   Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   non-targeting control shRNA embedded in miR30 backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pECE-M2-PAK2 S20A

   Plasmid

   #31681

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 S20A (PAK2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 20 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-p67MBP

   Plasmid

   #42

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   p67 myb binding protein (Mybbp1a Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pBlastR-5xUAS-sfGFP-GMRWhite

   Plasmid

   #165907

   Purpose

   Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable UAS Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-DNAJC5 (WT)-HaloTag

   Plasmid

   #205710

   Purpose

   Mammalian expression of DNAJC5 (WT)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

   Plasmid

   #171375

   Purpose

   For the mammalian expression of rat Gαi3 internally tagged with YFP

   Depositor

   Mikel Garcia-Marcos

   Article

   Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

   Insert

   Gai3-YFP
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3 Flag Jip1 JBD

   Plasmid

   #15751

   Purpose

   binds to Jnk

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted 1-126 and 282-660

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. PEAR1-bio-His

   Plasmid

   #51860

   Purpose

   Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PEAR1 (PEAR1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-HA-Nrxn1α-LNS2

    Plasmid

    #128106

    Purpose

    Express HA tagged Nrxn1α-LNS2

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS2 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-AMPK-β2

    Plasmid

    #40604

    Depositor

    Harvey Lodish

    Article

    Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

    Insert

    AMPK-β2 (Prkab2, Prkab2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-p160C

    Plasmid

    #46

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses aa580-1330

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

14. MBC1-PuroIII

    Plasmid

    #153419

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SCARF-bio-His

    Plasmid

    #51826

    Purpose

    Expresses full-length Endothelial cells scavenger receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SCARF (SCARF1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSL0373 (His-MBP-TniQ)

    Plasmid

    #130638

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-HA-Nrxn1α-LNS4

    Plasmid

    #128108

    Purpose

    Express HA tagged Nrxn1α-LNS4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS4 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-HA-Nrxn1α-LNS5

    Plasmid

    #128109

    Purpose

    Express HA tagged Nrxn1α-LNS5

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS5 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HSC1-D4Z4-GiP

    Plasmid

    #58542

    Purpose

    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-β-arrestin2 RRK/Q

    Plasmid

    #38261

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Arg233, Arg237, and Lys251 converted to Glutamines

    Promoter

    CMV

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

21. FLAG-TPD52

    Plasmid

    #172452

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SERPINC1-bio-His

    Plasmid

    #52076

    Purpose

    Expresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINC1 (SERPINC1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PTPRA_C433S-GFP fusion

    Plasmid

    #139638

    Purpose

    GFP-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA_C433S (PTPRA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C433S

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PTPRA_C723S-GFP fusion

    Plasmid

    #139639

    Purpose

    GFP-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA_C723S (PTPRA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C723S

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid

    #165910

    Purpose

    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-Nxph4-4Q-HA

    Plasmid

    #128104

    Purpose

    Express HA tagged Nxph4-4Q

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBacPAK8-His-myc-Rbx1

    Plasmid

    #29506

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Rbx1 (Rbx1 Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pGEX Nck-SH2

    Plasmid

    #46457

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nck (NCK1 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 275-377

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-Akt1(D323A/D325A)

    Plasmid

    #86630

    Purpose

    Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1(D323A/D325A) (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SRGN-bio-His

    Plasmid

    #52088

    Purpose

    Expresses full-length Serglycin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SRGN (SRGN Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET3a hRegIIIa

    Plasmid

    #64937

    Purpose

    bacterial expression of processed human RegIIIalpha

    Depositor

    Lora Hooper

    Article

    Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

    Insert

    RegIIIa (REG3A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    processed form. mutations made in 5' end to …

    Promoter

    T7

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pMA3379

    Plasmid

    #46874

    Purpose

    A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PLK3 335-646-pEGFP

    Plasmid

    #23267

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 335-646

    Promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

35. FUW-M2-PAK2 S20A

    Plasmid

    #31664

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 S20A (PAK2 Human)
    

    Use

    Lentiviral

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Changed Serine 20 to Alanine

    Promoter

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. C25-pLAC

    Plasmid

    #45283

    Purpose

    lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    pLAC-C25 (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    contains aa20-113 of lacritin

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SEMA4-bio-His

    Plasmid

    #51827

    Purpose

    Expresses full-length Semaphorin-4D precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SEMA4 (SEMA4D Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CFP-PBD(H83,86L)

    Plasmid

    #11402

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    p21-activated kinase 1 (PAK1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Obtained with Histidine residues 83 and 86 change…

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pBS/U6/RNAi SadBs shRNA mouse

    Plasmid

    #67152

    Purpose

    pBS/U6 containing shRNA to mouse SADBs

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    BRSK1 (Brsk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge