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Showing: 3801 - 3850 of 16714 results

  11. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  17. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  30. 6-ISG-IFN

    Plasmid
    #231488
    Purpose
    Standard for type I interferon signature (6 genes)
    Depositor
    Stefano Volpi
    Article
    Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d
    Inserts
    TBP (TBP Human)
    IFI27 (IFI27 Human)
    IFI44L (IFI44L Human)
    IFIT1 (IFIT1 Human)
    ISG15 (ISG15 Human)
    RSAD2 (RSAD2 Human)
    SIGLEC1 (SIGLEC1 Human)
    HPRT1 (HPRT1 Human)
    18s (RPS18 Human)
    G6PD (G6PD Human)
    Use
    Qpcr plasmid standard
    Tags
    Expression
    Mutation
    partial sequence
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231488-plasmid-map-sequence-id-469002

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  31. FR52re

    Plasmid
    #45489
    Depositor
    David Baker
    Insert
    FR52re
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    redesign of Di-I_5_FR28
    Promoter
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. PL2x2_5

    Plasmid
    #45491
    Depositor
    David Baker
    Insert
    PL2x2_5
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    changed E30Y of Di-IV_5_PL2x2_6
    Promoter
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. FR55

    Plasmid
    #45490
    Depositor
    David Baker
    Insert
    FR55
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    changed F14L and added Gly at N-terminus of Di-I_…
    Promoter
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pGLU_64

    Plasmid
    #225767
    Purpose
    This is the native promoter from the ermC erythromycin resistance cassette.
    Depositor
    Nili Ostrov
    Insert
    PermC_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225767-plasmid-map-sequence-id-446972

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  35. LHP1481 - TOPO-ProteinA

    Plasmid
    #32225
    Depositor
    Linda Hicke
    Insert
    Protein A
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    P-lac
    Available since
    Oct. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. pRSV-PKI-v2

    Plasmid
    #45066
    Purpose
    Expression of PKI (PKA inhibitor)
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RSV
    Available since
    July 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    45066-plasmid-map-sequence-id-62500

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  37. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

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  38. pKP2085

    Plasmid
    #167606
    Purpose
    Express ColicinB-V5-TEV-T7-mature MBP
    Depositor
    Kathleen Postle
    Insert
    cba-malE translational fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba ORF fused to V5 tag, TEV protease site, T7 ta…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167606-plasmid-map-sequence-id-335991

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  39. pET14b_tev_PDZ1

    Plasmid
    #59739
    Purpose
    expresses PDZ1 domain of human ZO-1 in bacteria
    Depositor
    Alan Fanning
    Insert
    zonula occludens-1 (TJP1 Human)
    Use
    Tags
    HIS tag and thrombin clvg
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pRSV-PKImut-v2

    Plasmid
    #45067
    Purpose
    Expression of intactive PKI (PKA inhibitor) mutant
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Arg 20 & 21 to Glycines
    Promoter
    RSV
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    45067-plasmid-map-sequence-id-62502

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  41. pLenti-hSyn1-mScarlet/CAAX

    Plasmid
    #197592
    Purpose
    Lentiviral transfer vector for the neuronal expression of membrane-bound mScarlet (good visualization of neuronal processes)
    Depositor
    Patrik Verstreken
    Insert
    mScarlet-CAAX
    Use
    Lentiviral
    Tags
    CAAX
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    197592-plasmid-map-sequence-id-393447

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  42. Nxn-1 beta Flag/pCMV

    Plasmid
    #197339
    Purpose
    Plasmid expressing an antigen targeting Neurexin 1beta
    Depositor
    Thomas Südhof
    Insert
    Neurexin 1beta (Nrxn1 Rat)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197339-plasmid-map-sequence-id-401579

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  43. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Pim Pijnappel
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159352-plasmid-map-sequence-id-314976

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  44. pLenti-hSyn1-eNpHR3.0-iRFP713

    Plasmid
    #197594
    Purpose
    Lentiviral transfer vector for the neuronal expression of eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal activity)
    Depositor
    Patrik Verstreken
    Insert
    eNpHR3.0-iRFP713
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197594-plasmid-map-sequence-id-389166

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  45. pLenti-hSyn1-mClover3/CAAX

    Plasmid
    #197591
    Purpose
    Lentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)
    Depositor
    Patrik Verstreken
    Insert
    mClover3-CAAX
    Use
    Lentiviral
    Tags
    CAAX
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197591-plasmid-map-sequence-id-389364

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  46. pLenti-hSyn1-TagBFP2/CAAX

    Plasmid
    #197590
    Purpose
    Lentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)
    Depositor
    Patrik Verstreken
    Insert
    mTagBFP2-CAAX
    Use
    Lentiviral
    Tags
    CAAX
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197590-plasmid-map-sequence-id-389168

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  47. pFGL1009

    Plasmid
    #119080
    Purpose
    Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).
    Depositor
    Naweed Naqvi
    Type
    Empty backbone
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119080-plasmid-map-sequence-id-232227

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  48. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid
    #197595
    Purpose
    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)
    Depositor
    Patrik Verstreken
    Insert
    mRuby2-T2A-GCaMP6f
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197595-plasmid-map-sequence-id-389365

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  50. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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Showing: 3801 - 3850 of 16714 results