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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Steven Clifford, Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG eGFP-Oct4

Plasmid

#203789

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou5f1 (Pou5f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP12-CB2x2-mTagBFP2-HAx2

Plasmid

#207146

Purpose

Tandem calcium-buffer with rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Calbindin-2 (CALB2 Human)

Tags

FKBP12, HA, and mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

Plasmid

#199368

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG eGFP-Oct6

Plasmid

#203790

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou3f1 (Pou3f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry-CD8-vhhGFP

Plasmid

#163930

Purpose

Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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PLK3 335-646-pEGFP

Plasmid

#23267

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 335-646

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
OVA-miSFIT-2xPerfect

Plasmid

#124673

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-5X2-hDrp1(518-736)-Flag

Plasmid

#37244

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Drp1 (DNM1L Human)

Tags

FLAG and GST

Expression

Bacterial

Mutation

contains AA 517-end

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only
AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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PLK3 1-334 T219D-pEGFP

Plasmid

#23268

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334 T219D

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
OVA-miSFIT-1xPerfect

Plasmid

#124674

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pADH99Cau

Plasmid

#244807

Purpose

Modified plasmid from pADH99 to perform HIS-FLP CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CauHIS1_US

Use

CRISPR

Tags

C. albicans MAL2 promoter, C. albicans codon opti…

Expression

Yeast

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5.1kh

Plasmid

#160297

Purpose

Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5.1nh

Plasmid

#160299

Purpose

Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5.1kn

Plasmid

#160298

Purpose

Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pILGFP4QE2Crimson

Plasmid

#218280

Purpose

Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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