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iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro

Plasmid

#204731

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BCR-ABL1-p190-IRES-EGFP

Plasmid

#219964

Purpose

Express human BCR-ABL1-P190 in mammalian cells

Depositor

Mohamed Fareh

Insert

BCR-ABL1-P190 (ABL1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p6351 MSCV-CMV-CMV-Flag-HA-NSD3

Plasmid

#31357

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

NSD3 (NSD3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Enzymatically inactive Cre

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

Plasmid

#209410

Purpose

Expression of wild-type neonatal isoform of human SCN2A.

Depositor

Al George

Article

Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

Insert

SCN2A Variant 4, stabilized (SCN2A Human)

Use

Tags

Expression

Mammalian

Mutation

IVS intron inserted after bp 4551 of the SCN2A OR…

Promoter

CMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

Plasmid

#214994

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV mLST8 myc (aka: GbL-myc)

Plasmid

#12580

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mLST8 (MLST8 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro

Plasmid

#204730

Purpose

Frt-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSF-1Rwt-IRES-GFP

Plasmid

#115523

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

human CSF-1 receptor (CSF1R Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

del52

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

Plasmid

#198508

Purpose

Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

CaMKIIα minimal promotor (0.4kb)

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

ins5

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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