We narrowed to 8,859 results for: sgRNA

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pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

Plasmid

#117409

Purpose

SauCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SauCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

Plasmid

#128760

Purpose

Expresses PARN gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PARN sgRNA #2 (PARN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

Plasmid

#239608

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEM040 [Multiome Perturb-seq sgRNA expression vector]

Plasmid

#224898

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

Depositor

Thomas Norman

Article

Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211692

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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